When we designed the laboratory may begin with the production of mycelium. It is important to know

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When we designed the laboratory may begin with the production of mycelium. It is important to know that in all phases of care on the part of sterility. In the air, on the ground, especially on the hands and mouth teeming perforating tool with bacteria, spores, viruses and organisms of all kinds. Each of these impurities uncompromising us destroy the whole effort. To maintain a sterile environment during work using isopropanol and hydrogen pereoksid. To sterilize jars and petri dishes economist pot and oven. For sterile perforating tool scalpel, the alcohol burner. perforating tool
Known are two ways to reproduce the mycelium. The first is from tissue culture (cloning), the second Reproduction of spores (sexual perforating tool reproduction). For larger mushrooms are commonly makes use of reproductive tissue, which I will try to describe as accurately as possible.
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Petri dishes (of 5 total) hermetically wrapped in aluminum foil and place in oven at 200 degrees. After 20 minutes, the oven switched off and allowed perforating tool to cool as it. In the meantime, Cook agar, which is the most nutritious perforating tool substance for the initial perforating tool growth of the mycelium from the tissue. Peel 300g of potatoes and cut into slices. Cook it in a liter of water an hour, wait for it to cool and strain through perforating tool a fine cloth. In the resulting potato water, stir in 10g and 20g glucose Agra (agar agar). The resulting liquid poured into a glass that is temperature resistant. Personally, I use one 800ml, which I buy in the store with the goods, chemical-glass ceramic. The glass should be filled up to two thirds. Cover it with a thin cloth over it pulled aluminum foil and sealed with a rubber band. Place the pot in economic, but not at the bottom. It is best to put on the bottom of the dish. If the glass is at the bottom of the agar may zasmodi.Vode the pot should be about 3 cm. Cook under pressure for half an hour, and then wait for about two to three hours to cool the pot. After cooking, do not release steam by hand, because there may be a low pressure and give you the ambient air is pulled into the agar.
When we assess perforating tool the agar cooled, it's time for the preparation of the surfaces are included. Turn on the fan and clean work surface (windows) upstream of the filter with isopropanol. I use cloth which I use only for this purpose, and after work always clean-wash.
Take out of the oven wrapped in foil plates. Before we give roll on the work surface disinfect it with izoprapanolom. By disinfected perforating tool their hands before you unscrew the filter and petri dishes stacked on the left side of the work surface (if you are left-handed to right). The procedure was repeated with a glass in which the agar, with the difference that only the place to the right (for ease of handling).
Again disinfect hands. perforating tool With one hand, lift the lid of the Petri dish, with the other hand from the glass poured into petri dishes of agar. Petri dish sealed with a lid and place it on the side surfaces (still in a sterile work environment). The thickness of Agra in the petri dish should be between 4-5mm. Once you fill all the Petri dish, you can relax a little.
Completion of the first phase represents perforating tool the input tissue in agar. Sponge with a scalpel in the stalk is cut longitudinally an inch, then with his hands before the filter in half so that the middle part of the mushrooms constantly facing the filter (drafts). It is a fact that the fungus perforating tool in its internal part is essentially sterile and protected from contamination. This part - the meat at the top of the beta transferred to agar. Above the burner holds the knife until shines. Allow to cool before the filter (approx. 20s) and from said part of the cut out piece of sponge (about 2 times 2 mm). With the tip of a scalpel, this piece is transferred to the center of the Petri dish (Agra). On the petri dish lid pulled and placed on the page work surface. Once we vaccinate all the dishes take in hands roll of film for the food, we take care petri dish and close at hand turn. The foil has such a function that breath, it is too thick, it would be descended through the self bacteria and infection.
Petri dishes placed in a dark incubator at an average temperature of 25 degrees Celsius. After about 4-5 days spawn (like a warbler) turns a piece of sponge, then starts spreading citrus fruits. At the right temperature, the dish is overgrown in 10 to 14 days. In the meantime, we observe mycelial growth and keep removing the Petri dish, in which we observed a possible infection. Growing mushrooms on March 25, 2013 by admin_ekogea. Navigation contributions My experience with donkeys Organic Farm In Jernejk
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